Related Experiment Video

Updated: May 3, 2026

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

13.1K

Complete Genome Sequence of the Pseudomonas aeruginosa Bacteriophage phiIBB-PAA2

Diana P Pires1, Andrew M Kropinski, Joana Azeredo

  • 1Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal.

Genome Announcements
|February 8, 2014
PubMed

Insights

A novel Pseudomonas aeruginosa phage, phiIBB-PAA2, was identified in hospital sewage. This broad-host-range virus possesses a large double-stranded DNA genome and belongs to the Podoviridae family.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Pseudomonas aeruginosa is an opportunistic pathogen.
  • Bacteriophages are viruses that infect bacteria and are potential therapeutic agents.
  • Characterization of novel phages is crucial for understanding phage-host interactions and developing phage therapy.

Purpose of the Study:

  • To isolate and characterize a novel bacteriophage infecting Pseudomonas aeruginosa.
  • To determine the genomic features and taxonomic classification of the isolated phage.

Main Methods:

  • Isolation of bacteriophage from raw hospital sewage.
  • Genome sequencing and analysis of the double-stranded DNA (dsDNA) genome.
  • Bioinformatic analysis to identify coding sequences (CDSs) and tRNAs.
  • Phylogenetic analysis for taxonomic classification.

Main Results:

  • Isolation of a broad-host-range Pseudomonas aeruginosa phage, designated phiIBB-PAA2.
  • The phage has a 45,344 bp dsDNA genome with terminally redundant sequences.
  • The genome encodes 66 coding sequences (CDSs) and 3 tRNAs.
  • Phylogenetic analysis places phiIBB-PAA2 within the Podoviridae family, genus Luz24likevirus.

Conclusions:

  • Pseudomonas aeruginosa phage phiIBB-PAA2 is a newly identified member of the Podoviridae family.
  • Its broad-host-range and genomic characteristics warrant further investigation for potential applications in phage therapy.

More Related Videos

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

7.6K
Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi09:01

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

Published on: September 28, 2022

2.5K

Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.6K
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
60.2K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
49.8K
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
3.0K