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Updated: May 3, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
Nuclear division in isolated protoplasts from cells of higher plants grown in vitro
1Institute of Physiological Botany, University of Uppsala, Sweden.
Abstract:
Protoplasts were isolated from cell suspensions of Haplopappus gracilis. The cell walls were degraded by the cellulase preparation "Onozuka P 1500" at a concentration of 5%. Sorbitol was found to work well as osmotic stabilizer in concentrations of 0.4-0.6 M. The protoplasts were cultured in growth medium after isolation; 3-5% went through nuclear division once and less than 1% also for a second time. No nuclear fusion was observed.
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