Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oxygenic Photosynthesis01:26

Oxygenic Photosynthesis

994
Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate...
994
The Z-Scheme of Electron Transport in Photosynthesis01:34

The Z-Scheme of Electron Transport in Photosynthesis

12.6K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
12.6K
Anoxygenic Photosynthesis01:30

Anoxygenic Photosynthesis

1.9K
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
1.9K
Photosystem II01:22

Photosystem II

59.9K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across  two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
59.9K
Photosystem I01:27

Photosystem I

52.8K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
52.8K
Chemiosmosis01:32

Chemiosmosis

84.9K
Oxidative phosphorylation is a highly efficient process that generates large amounts of adenosine triphosphate (ATP), the basic unit of energy that drives many cellular processes. Oxidative phosphorylation involves two processes— the electron transport chain and chemiosmosis.
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
84.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Respiration induced by blue light.

Planta·2014
Same author

Revival of respiration and photosynthesis in dried leaves of Polypodium polypodioides.

Planta·2014
Same author

Transient color sensitivity of the hill reaction during the disintegration of chloroplasts.

Planta·2014
Same author

Photoproduction of hydrogen by photosystem I of Scenedesmus.

Planta·2014
Same author

Transient light effects in the Hill reaction of disintegrating chloroplasts in vitro.

Planta·2014
Same author

Hydrogen production by photosystem I of Scenedesmus: Effect of heat and salicylaldoxime on electron transport and photophosphorylation.

Planta·2014
Same journal

Regulatory roles of R2R3-MYB genes in plant growth, development and stress adaptation: insights into seed dormancy and germination.

Planta·2026
Same journal

Assembly and comparative analysis of the complete mitochondrial genome of Viola philippica (Malpighiales, Violaceae).

Planta·2026
Same journal

Somatic embryogenesis-induced epigenetic changes promoting catechin accumulation in Vaccinium vitis-idaea L.

Planta·2026
Same journal

Integrative transcriptome and long non-coding RNA analysis to decipher the molecular basis of cleistogamy in pigeonpea (Cajanus cajan (L) Millsp).

Planta·2026
Same journal

RDO3 REPRESSOR 27, a new MED25 allele, regulates seed dormancy dependent on DOG1 and ABA pathways in Arabidopsis.

Planta·2026
Same journal

Development of subtropically-adapted indeterminate gametophyte1 (ig1) gene-based paternal haploid inducer lines in maize through molecular breeding.

Planta·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
10:21

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

7.5K

Light-dependent hydrogen evolution by Scenedesmus.

H Kaltwasser1, T S Stuart, H Gaffron

  • 1Institute of Molecular Biophysics, Florida State University, Tallahassee.

Planta
|February 8, 2014
PubMed
Summary
This summary is machine-generated.

Hydrogen production in Scenedesmus obliquus D3 algae is enhanced by glucose and the uncoupler Cl-CCP. This process relies on organic material degradation via the Embden-Meyerhof pathway, independent of oxygen production by photosystem II.

More Related Videos

Experimental Methods for Efficient Solar Hydrogen Production in Microgravity Environment
11:38

Experimental Methods for Efficient Solar Hydrogen Production in Microgravity Environment

Published on: December 3, 2019

7.2K
Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
07:10

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Published on: February 3, 2023

1.5K

Related Experiment Videos

Last Updated: May 3, 2026

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
10:21

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

7.5K
Experimental Methods for Efficient Solar Hydrogen Production in Microgravity Environment
11:38

Experimental Methods for Efficient Solar Hydrogen Production in Microgravity Environment

Published on: December 3, 2019

7.2K
Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
07:10

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Published on: February 3, 2023

1.5K

Area of Science:

  • * Biochemistry and Photosynthesis
  • * Microbial Hydrogen Production
  • * Algal Metabolism

Background:

  • * Understanding hydrogen (H2) production in algae is crucial for developing sustainable energy sources.
  • * The role of specific metabolic pathways and cellular conditions in algal H2 evolution requires further elucidation.

Purpose of the Study:

  • * To investigate the effects of glucose and the uncoupler carbonyl cyanide m-chlorophenylhydrazone (Cl-CCP) on H2 production in adapted Scenedesmus obliquus D3 cells.
  • * To determine the metabolic pathways involved in light-dependent H2 evolution.
  • * To assess the necessity of oxygen production by photosystem II for H2 evolution.

Main Methods:

  • * Experiments conducted on adapted Scenedesmus obliquus D3 cells.
  • * Application of varying concentrations of Cl-CCP and glucose.
  • * Utilized specifically labeled glucose ((14)CO2) to trace metabolic degradation pathways.
  • * Tested a mutant strain (No. 11) unable to evolve oxygen.

Main Results:

  • * Cl-CCP (10(-5)M) inhibited dark H2 evolution and increased light H2 evolution, with temporary inhibition of photosynthesis and photoreduction.
  • * Higher Cl-CCP concentration (5 x 10(-5)M) maximized photohydrogen production while inhibiting other processes.
  • * H2 evolution correlated with CO2 release, indicating organic matter degradation via the Embden-Meyerhof pathway.
  • * Glucose addition or heterotrophic growth stimulated H2 production; starvation reduced it.
  • * An oxygen-evolving deficient mutant showed no inhibition in light-induced H2 production.

Conclusions:

  • * Light-dependent H2 evolution in adapted Scenedesmus obliquus is dependent on the degradation of organic compounds.
  • * The Embden-Meyerhof pathway is involved in the breakdown of glucose for H2 production.
  • * Oxygen evolution by photosystem II is not required for H2 production in the light.