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Related Experiment Video

Updated: May 3, 2026

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

Genome-scale transcript analyses with human neutrophils.

Scott D Kobayashi¹, Daniel E Sturdevant, Frank R Deleo

¹Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 8, 2014

Summary

This summary is machine-generated.

This study presents a standardized method for analyzing gene expression in human neutrophils using oligonucleotide microarrays. These methods provide new insights into resolving infections by examining polymorphonuclear leukocyte transcript levels.

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Last Updated: May 3, 2026

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High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Area of Science:

Molecular Biology
Immunology
Genomics

Background:

Transcriptome analyses have advanced understanding of biological and pathological processes.
Gene expression studies in polymorphonuclear leukocytes (PMNs) offer insights into human infection resolution.

Purpose of the Study:

To present standardized methods for analyzing gene expression in human neutrophils.
To facilitate evaluation of PMN transcript levels using commercially available materials.

Main Methods:

Isolation of high-quality RNA from human neutrophils.
Generation and labeling of complementary RNA (cRNA).
Affymetrix oligonucleotide microarray hybridization and scanning.

Main Results:

The described procedures utilize readily available reagents and materials.
This represents a standardized approach for evaluating PMN transcript levels.
The methods enable global-scale analysis of neutrophil gene expression.

Conclusions:

The presented methods offer a standardized approach for human neutrophil transcriptome analysis.
This facilitates research into the molecular mechanisms of infection resolution.
Standardized methods are crucial for reproducible and comparable gene expression studies.