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Nucleotide sequence of cloned cDNA coding for pumpkin 11-S globulin beta subunit
M Hayashi1, H Mori, M Nishimura
1Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Kobe, Japan.
European Journal of Biochemistry
|March 15, 1988
Summary
Researchers cloned cDNA for preproglobulin beta, a precursor to the 11-S globulin beta subunit. The nucleotide sequence reveals the protein
Area of Science:
- Molecular Biology
- Protein Chemistry
- Plant Biochemistry
Background:
- 11-S globulins are major storage proteins in legumes and other plants.
- Understanding their precursor proteins is crucial for seed development and protein structure studies.
Purpose of the Study:
- To clone and determine the nucleotide sequence of cDNA encoding preproglobulin beta.
- To analyze the deduced amino acid sequence and compare it with other 11-S globulin precursors.
Main Methods:
- cDNA cloning and nucleotide sequencing.
- Amino acid sequence analysis using dot matrix analysis.
Main Results:
- The complete coding sequence (1440 bp) and flanking noncoding regions of preproglobulin beta cDNA were determined.
- The deduced amino acid sequence revealed a signal peptide, an acidic gamma region, and a basic delta region.
- Post-translational cleavage site and the N-terminal pyroglutamic acid residue of the mature gamma chain were identified.
Conclusions:
- The determined sequence provides insights into the structure and processing of 11-S globulin beta subunits.
- Comparative analysis suggests evolutionary relationships among different plant 11-S globulins.