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Concentration change of TGF -β 1 in aqueous humor of rabbits
Xiao-Yi Yu1, Wei Wu2, Xiao-He Lu2
1Department of Ophthalmology, Southern Medical University, Guangzhou 510282, Guangdong Province, China; Department of Ophthalmology, Wuyi Traditional Chinese Medicine Hospital, Jiangmen, Guangdong Province, China.
Objective:
To observe the influence of the the transforming growth factor β1 (TGF-β1) eye drops on rabbit aqueous humor TGF-β1 concentration, and to analyze the best drug concentration.
Methods:
A total of 30 New Zealand white rabbits were randomly divided into 5 groups with 6 in each. Rabits in control group had PBS eye drops, group A, B, C, D adopted TGF-β1 eye drops at 0.5, 1.0, 2.0, 4.0 mg/L, respectively, 4 times a day. Aqueous humor of right eye was extracted 1 week after administration to detect concentration changes of TGF-β1 by ELISA; rabbits in fpur hroups adopted 2.0 mg/L eye drops to left eyes 4 times a day, 0.2 mL aqueous humor was extracted left eye at the scheduled time point 0, 30 min, 2 h, 4 h, 24 h for testing, the slit lamp was used to observe the cornea, chamber and lens.
Results:
No obvious pathological changes in conjunctiva, cornea, rabbit conjunctival, anterior chamber, and the lens was found. Concentration of TGF-β1 in rabbit aqueous humor in C, D group was significantly higher than the control group (P<0.05).
Conclusions:
TGF-β1 eye drops at 2.0 mg/L, 4.0 mg/L can significantly increase concentration of TGF-β1 in rabbit aqueous humor, withe good ocular surfac permeability.
