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The regulatory impact of immune inhibitors on T cells of SD rats
Chao-Hua Zhang1, Yan Huang2, Gang Han3
1Uropoiesis Surgical Department, First Hospital of Baoding, Hebei, China; Medicine Clinical, Hebei University College, Hebei, China.
Objective:
To observe the regulatory impact of immune inhibitors on T cells in rats.
Method:
Forty SD rats were selected and randomly divided into experimental group and control group, Rapamycin (SRL) 0.4 mg/d to fill the stomach of the former one, saline lavage was used with the latter one for two weeks. Using flow cytometry to detect the two groups of rats with spleen and thymus level of CD4+ CD25+ T cells; and the spleen cells FoxP3 mRNA expression; Using ELISA method to detect TGF-β, IL-10 levels.
Results:
The peripheral blood, spleen and thymus of CD4+ CD25+ T cells accounted for the proportion of mononuclear cells were significantly higher than that of control group (P<0.05); FoxP3 mRNA expression quantity also significantly higher than the control group (P<0.05); Experimental TGF-β in rats, IL-10 levels are significantly higher than control group (P<0.05).
Conclusions:
Immune inhibitors can regulatory CD4+ CD25+ foxp3+ T cells in rats, a single nuclear cell proportion increase, shows that it can induce rat CD4+ CD25+ foxp3+ regulatory T cells proliferation.
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