Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single channel recording and gating function of ionic channels.

F Franciolini1, A Petris

  • 1Department of Physiology and Biophysics, University of Miami School of Medicine, Florida 33101.

Experientia
|March 15, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Multi-Scale Approach to Model K<sup>+</sup> Permeation Through the KcsA Channel.

Frontiers in molecular biosciences·2022
Same author

Electrocardiographic changes in the most frequent endocrine disorders associated with cardiovascular diseases. Review of the literature.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2015
Same author

Lasing in DNA-CTMA doped with Rhodamine 610 in butanol.

Physical chemistry chemical physics : PCCP·2015
Same author

Barriers of antiaggregant treatment.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2014
Same author

Amiodarone-induced thyroid dysfunction--clinical picture. Study on 215 cases.

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi·2014
Same author

Effect of paradoxical sleep deprivation on dna synthesis in fetal rat brain.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2014

This review details kinetic analysis of membrane channel gating using single-channel patch clamp data. It covers state estimation, state lifetimes influenced by voltage or ligands, and transition rates for understanding channel mechanisms.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Biochemistry

Background:

  • Membrane channels are crucial for cellular function, regulating ion and molecule transport.
  • Understanding channel gating mechanisms is key to deciphering cellular signaling and disease pathologies.

Purpose of the Study:

  • To provide a comprehensive review of kinetic analysis methods for membrane channel gating.
  • To detail the estimation of channel states, state lifetimes, and transition rates from single-channel patch clamp data.

Main Methods:

  • Analysis of single-channel recordings obtained via the patch clamp technique.
  • Kinetic modeling to estimate the number of channel conformations.
  • Determination of state lifetimes as a function of transmembrane voltage or ligand concentration.

Related Experiment Videos

Main Results:

  • Methods for estimating the number of distinct channel conformations are discussed.
  • The dependence of state lifetimes on voltage (for voltage-gated channels) and ligand concentration (for ligand-gated channels) is reviewed.
  • Techniques for estimating transition rates between channel states and assessing reaction mechanisms are presented.

Conclusions:

  • Kinetic analysis of single-channel data provides powerful insights into membrane channel function.
  • Understanding channel gating kinetics is essential for fields ranging from basic science to drug development.
  • This review synthesizes current approaches for analyzing complex channel gating behaviors.