Optimized extraction of polysaccharides from Cymbopogon citratus and its biological activities
Ramar Thangam1, Veeraperumal Suresh2, Soundarapandian Kannan3
1Proteomics & Molecular Cell Physiology Lab (PMCPL), Department of Zoology, Bharathiar University, Coimbatore, TN, India; Department of Virology, King Institute of Preventive Medicine and Research, Chennai, TN, India.
Abstract:
In this study the extraction of hot water soluble polysaccharides (HWSPs) from Cymbopogon citratus using hot water decoction was discussed. Response surface methodology (RSM) based on a three level, three variable central composite rotatable design (CCRD), was employed to obtain best possible combination of extraction time (X1: 30-180 min), extraction temperature (X2: 70-100 °C) and water to the raw material ratio (X3: 10-60) for maximum HWSPs extraction. The optimum extraction conditions were as follows: extraction time was around 113.81 min, extraction temperature at 99.66 °C and the ratio of water to raw material was 33.11 g/mL. Under these conditions, the experimental yield was 13.24±0.23%, which is well in close agreement with the value predicted by RSM model yield (13.19%). The basic characterization of HWSPs was determined by using the FTIR. These preliminary in vitro biological studies indicated that lemongrass polysaccharides were useful for anticancer therapy.
