Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vitro Drug Release Testing: Overview, Development and Validation01:10

In Vitro Drug Release Testing: Overview, Development and Validation

593
In vitro dissolution and drug release tests assess how quickly and how much of a drug is released from its dosage form into an aqueous medium under standardized laboratory conditions. These tests are essential tools in pharmaceutical development and quality assurance, offering insight into the drug's performance before clinical use.During formulation development, dissolution testing identifies incomplete or inconsistent drug release issues. It also supports decisions on selecting the optimal...
593
In vitro Mutagenesis01:16

In vitro Mutagenesis

4.9K
4.9K
Toxicity Testing in Animals01:23

Toxicity Testing in Animals

204
Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
204
In Vitro Drug Dissolution: Compendial Testing Models II01:09

In Vitro Drug Dissolution: Compendial Testing Models II

686
Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients,...
686
In Vitro Drug Dissolution: Compendial Testing Models I01:13

In Vitro Drug Dissolution: Compendial Testing Models I

560
Compendial dissolution methods are standardized procedures defined by pharmacopeias to evaluate the rate at which a drug dissolves in a specific medium. These methods ensure batch-to-batch consistency, enable quality control, and support the prediction of drug bioavailability. They are critical for both immediate and modified-release drug products.The apparatuses used for dissolution testing differ in their design and mechanical function, but all aim to simulate the physiological environment of...
560
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a capsule phase microextraction (CPME) protocol for the determination of four tetracyclines in human urine prior to HPLC-DAD analysis.

Analytical and bioanalytical chemistry·2026
Same author

Application of the click analytical chemistry index for the assessment of endocrine-disrupting chemicals in cosmetics and personal care products.

Journal of chromatography. A·2026
Same author

An efficient fabric phase sorptive extraction protocol combined with UPLC-MS/MS for the trace-level determination of PFAS in sunflower oil.

Journal of chromatography. A·2025
Same author

Artificial Intelligence as a Scientific Copilot in Analytical Chemistry: Transforming How We Write, Review, and Publish.

Analytical chemistry·2025
Same author

Violet Innovation Grade Index (VIGI): A New Survey-Based Metric for Evaluating Innovation in Analytical Methods.

Analytical chemistry·2025
Same author

Editorial: Application of metabolomics, peptidomics and proteomics in human nutrition and health.

Frontiers in chemistry·2024

Related Experiment Video

Updated: May 3, 2026

A Small-Scale Setup for Algal Toxicity Testing of Nanomaterials and Other Difficult Substances
08:18

A Small-Scale Setup for Algal Toxicity Testing of Nanomaterials and Other Difficult Substances

Published on: October 10, 2020

5.2K

Youden test application in robustness assays during method validation.

Eftichia Karageorgou1, Victoria Samanidou1

  • 1Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.

Journal of Chromatography. A
|February 11, 2014
PubMed
Summary

The Youden test, using fractional factorial designs, minimizes experiments for analytical method validation robustness testing. This approach saves time and resources while ensuring reliable method performance.

Keywords:
Method validationRobustnessRuggednessYouden test

More Related Videos

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

10.3K
A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
13:22

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Published on: September 10, 2013

13.3K

Related Experiment Videos

Last Updated: May 3, 2026

A Small-Scale Setup for Algal Toxicity Testing of Nanomaterials and Other Difficult Substances
08:18

A Small-Scale Setup for Algal Toxicity Testing of Nanomaterials and Other Difficult Substances

Published on: October 10, 2020

5.2K
Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

10.3K
A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
13:22

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Published on: September 10, 2013

13.3K

Area of Science:

  • Analytical Chemistry
  • Method Validation
  • Quality Control

Background:

  • Analytical method validation is crucial for reliable performance.
  • Robustness/ruggedness studies assess method stability under varying conditions.
  • Traditional robustness testing is time-consuming and resource-intensive.

Purpose of the Study:

  • To review the Youden test as an efficient approach for robustness studies.
  • To highlight the advantages of the Youden test in minimizing experimental effort.
  • To discuss typical applications of this robustness testing method.

Main Methods:

  • The Youden test, employing fractional factorial designs.
  • Limited number of determinations using combinations of investigated factors.
  • Review of literature applications across diverse sample matrices.

Main Results:

  • The Youden test significantly reduces the number of experiments required.
  • It minimizes time and consumable usage for robustness testing.
  • Demonstrated effectiveness across various analytical applications.

Conclusions:

  • The Youden test is a highly effective and efficient method for robustness testing in analytical validation.
  • It offers a practical solution to the resource demands of traditional methods.
  • Its application is versatile across different sample types and analytical processes.