Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Histamine release from human leukocytes by platelet-activating factor.

Y Okuda1, I Tsuyuguchi, A Yamatodani

  • 1Osaka Prefectural Habikino Hospital, Japan.

International Archives of Allergy and Applied Immunology
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulation of basophilic and erythroid-differentiation of a human chronic myelogenous leukemia-cell line, ku812f, by interleukin-3 and stem-cell factor.

International journal of oncology·2011
Same author

Relationship between the isoniazid-resistant mutation katGS315T and the prevalence of MDR-/XDR-TB in Osaka, Japan.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2008
Same author

The antiarrhythmic effect of centrally administered rilmenidine involves muscarinic receptors, protein kinase C and mitochondrial signalling pathways.

British journal of pharmacology·2008
Same author

A possible mechanism of alpha-fluoromethylhistidine-induced increase of food intake.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2004
Same author

Thioperamide-elicited increase of histamine release from basolateral amygdala of freely moving rats and its therapeutic implications.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2004
Same author

The role of the chorda tympani nerve and histaminergic system in TCDD toxicity.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2004

Platelet-activating factor (PAF) triggers histamine release from human basophils, independent of IgE. This PAF-induced histamine release is elevated in allergic asthmatics, offering insights into allergic disease mechanisms.

Area of Science:

  • Immunology
  • Allergology
  • Pharmacology

Background:

  • Platelet-activating factor (PAF) is a potent inflammatory mediator implicated in allergic reactions.
  • Basophils are key effector cells in allergic responses, releasing histamine and other mediators.
  • Understanding PAF's role in basophil activation is crucial for developing targeted therapies for allergic disorders.

Purpose of the Study:

  • To investigate the ability of Platelet-activating factor (PAF) to induce histamine release from human basophils.
  • To characterize the mechanism and conditions of PAF-induced histamine release.
  • To explore the potential of PAF-induced histamine release as a biomarker in allergic diseases.

Main Methods:

  • Human basophils in mixed leukocytes were incubated with Platelet-activating factor (PAF) in the presence of cytochalasin B.

Related Experiment Videos

  • Histamine release was measured under varying PAF concentrations and temperatures.
  • The effects of EDTA and a specific PAF antagonist (CV-3988) were evaluated.
  • Comparisons were made between leukocytes from allergic asthmatics and healthy controls.

    • Main Results:

    • Platelet-activating factor (PAF) induced rapid, temperature-dependent histamine release from basophils.
    • Histamine release showed a non-linear dose-response to PAF and was inhibited by EDTA, suggesting a noncytolytic mechanism.
    • A PAF antagonist (CV-3988) inhibited the release in a dose-dependent manner.
    • PAF-induced histamine release was significantly higher in allergic asthmatics compared to controls, irrespective of IgE levels.

    Conclusions:

    • Platelet-activating factor (PAF) is a potent stimulator of histamine release from human basophils via a noncytolytic pathway.
    • The elevated PAF-induced histamine release in allergic asthmatics highlights PAF's role in the pathogenesis of allergic disorders.
    • PAF-induced histamine release serves as a valuable experimental model for studying allergic inflammation and evaluating therapeutic interventions.