Related Experiment Video

Updated: May 3, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

9.3K

DNA complexed with TAT peptide and condensed using calcium possesses unique structural features compared to PEI

Abdulgader A Baoum1, C Russell Middaugh2, Cory Berkland3

  • 1Department of Chemistry, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, KS 66047, USA.

International Journal of Pharmaceutics
|February 11, 2014
PubMed
Summary

TAT peptide complexes with plasmid DNA (pDNA) exhibit high transfection efficiency. Structural analysis revealed pDNA remains in B conformation, with TAT stabilizing it more than PEI, though direct correlation to efficiency was unclear.

Keywords:
Biophysical characterizationGene deliveryPolyelectrolyte complexes

More Related Videos

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.5K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.5K

Related Concept Videos

DNA Packaging00:58

DNA Packaging

Overview
94.6K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
45.9K
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.1K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
12.9K
Condensins02:15

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.7K
Condensins02:15

Condensins

1.8K