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Purification of a High Molecular Mass Protein in Streptococcus mutans
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β-Phosphoglucomutase contributes to aciduricity in Streptococcus mutans.

Andrew A Buckley1, Roberta C Faustoferri2, Robert G Quivey2,1

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Microbiology (Reading, England)
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Streptococcus mutans uses pgmB to convert β-d-glucose 1-phosphate to glucose 6-phosphate. Deleting pgmB reduces acid tolerance and virulence in this oral bacterium.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Oral Health

Background:

  • Streptococcus mutans metabolizes dietary sugars in the oral cavity.
  • β-d-glucose 1-phosphate (βDG1P) is converted to glucose 6-phosphate (G6P) for metabolism.
  • β-phosphoglucomutase (pgmB) mediates this conversion, previously identified in Lactococcus lactis.

Purpose of the Study:

  • To identify and designate the β-phosphoglucomutase gene in Streptococcus mutans.
  • To investigate the role of pgmB in S. mutans virulence, focusing on acid tolerance and metabolism.
  • To assess the impact of pgmB deletion on S. mutans virulence in a model organism.

Main Methods:

  • Identification of the pgmB orthologue (SMU.1747c) in S. mutans.
  • Construction and characterization of a ΔpgmB deletion strain.
  • Assessment of acid tolerance, glycolytic profiles, and Galleria mellonella infection model.

Main Results:

  • SMU.1747c was confirmed as the functional β-phosphoglucomutase gene (pgmB) in S. mutans.
  • The ΔpgmB strain exhibited reduced ability to convert βDG1P to G6P.
  • Deletion of pgmB led to decreased acid tolerance, diminished glycolysis, and reduced virulence in the Galleria mellonella model.

Conclusions:

  • pgmB is essential for βDG1P metabolism in S. mutans.
  • pgmB plays a significant role in S. mutans acid tolerance and virulence.
  • The pgmB gene links carbohydrate metabolism to the virulence of S. mutans.