A mass spectrometry-based hybrid method for structural modeling of protein complexes
Argyris Politis1, Florian Stengel2, Zoe Hall1
1Department of Chemistry, University of Oxford, South Parks Road, Oxford, United Kingdom.
Abstract:
We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.
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