Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Profiling Protein Aggregate Size Using Single-Molecule Array Technology.

Analytical chemistryĀ·2026
Same author

Divergent toxicity mechanisms of amyloid-beta aggregates arising from a single aggregation reaction.

Cell reportsĀ·2026
Same author

PD-1 signaling and PD-1 blockade-mediated tumor control are established at microvillar T cell contacts.

Science immunologyĀ·2026
Same author

Single-molecule detection methods to study alpha-synuclein aggregation in postmortem Parkinson's disease brains.

Cell reports methodsĀ·2026
Same author

A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)Ā·2026
Same author

Cortisol-resistant CAR-NK cells overcome steroid-induced immunosuppression in lung cancer.

Signal transduction and targeted therapyĀ·2026
Same journal

Altered expression of Toll-like receptorĀ 9 in the lung tissue of adult mice generated by in vitro embryo culture and embryo transfer.

Histochemistry and cell biologyĀ·2026
Same journal

Dynamic changes in OTULIN and progranulin levels in experimental myocardial infarction and cardiac remodeling.

Histochemistry and cell biologyĀ·2026
Same journal

Eosinophil-associated matrix remodeling in a sterile granulomatous inflammation model: a temporal histopathological analysis.

Histochemistry and cell biologyĀ·2026
Same journal

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biologyĀ·2026
Same journal

From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.

Histochemistry and cell biologyĀ·2026
Same journal

Immunohistochemical study of Ī±-keratin, loricrin, filaggrin-like protein, and transglutaminase-1 expression in orthokeratinized and parakeratinized epithelium of the tongue of domestic goose (Anser anser f. domestica) during embryonic development.

Histochemistry and cell biologyĀ·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.4K

The changing point-spread function: single-molecule-based super-resolution imaging.

Mathew H Horrocks1, Matthieu Palayret, David Klenerman

  • 1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.

Histochemistry and Cell Biology
|February 11, 2014
PubMed
Summary
This summary is machine-generated.

Super-resolution imaging techniques overcome diffraction limits, enabling new studies in condensed phase biology. This review covers principles, recent advances, and applications of these powerful microscopy methods.

More Related Videos

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

773
Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

775

Related Experiment Videos

Last Updated: May 3, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.4K
Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

773
Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

775

Area of Science:

  • Optical microscopy
  • Biophysics
  • Cell biology

Background:

  • Traditional fluorescence microscopy is limited by diffraction.
  • Advancements are needed to study biological systems at higher resolution.
  • Super-resolution imaging offers a solution to overcome these limitations.

Purpose of the Study:

  • To review the principles of super-resolution imaging.
  • To summarize recent developments in the field.
  • To provide examples of its application in biological research.

Main Methods:

  • Explanation of 2D and 3D super-resolution imaging principles.
  • Summary of key technological advancements.
  • Case studies of biological applications.

Main Results:

  • Super-resolution microscopy enables visualization beyond the diffraction limit.
  • Diverse techniques have been developed over the past decade.
  • These methods have facilitated the study of complex biological systems.

Conclusions:

  • Super-resolution imaging is a transformative tool in biological sciences.
  • It allows unprecedented insights into cellular structures and dynamics.
  • Continued development promises further breakthroughs in understanding life at the nanoscale.