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In vitro and transdermal penetration of PHBV micro/nanoparticles
G Eke1, A M Kuzmina, A V Goreva
1Department of Micro and Nanotechnology, Middle East Technical University, 06800, Ankara, Turkey.
Journal of Materials Science. Materials in Medicine
|February 11, 2014
Summary
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) micro/nanoparticles were developed for drug delivery. Studies showed these PHBV particles are non-toxic and can penetrate skin cells, indicating potential for topical and transdermal applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Pharmacology
Background:
- Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a biodegradable polymer with potential applications in drug delivery.
- Developing effective micro and nano-sized carriers is crucial for enhancing drug efficacy and targeted delivery.
- Understanding particle-cell and particle-skin interactions is essential for evaluating drug carrier safety and performance.
Purpose of the Study:
- To synthesize and characterize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) micro and nanospheres.
- To investigate the in vitro cell penetration and viability of PHBV micro/nanospheres.
- To evaluate the in vivo transdermal permeation and skin tissue reaction to PHBV micro/nanospheres.
Main Methods:
- PHBV micro/nanospheres were prepared using the o/w emulsion method and fractionated by centrifugation.
- Particle size, distribution, and topography were analyzed using particle sizer and SEM.
- In vitro cytotoxicity was assessed using MTT assay on L929 cells, and cell penetration was studied.
- In vivo studies involved transdermal permeation and histological evaluation in a BALB/c mouse model, with PHBV quantification by GC-MS.
Main Results:
- PHBV micro/nanospheres with average diameters of 1.9 μm, 426 nm, and 166 nm were successfully prepared.
- In vitro studies indicated no cytotoxicity, with normal cell growth observed.
- Nanoscale PHBV spheres (426 nm and 166 nm) demonstrated penetration of the cell membrane.
- In vivo histological examination showed no adverse tissue reactions, and PHBV was detected in the skin.
Conclusions:
- PHBV micro/nanoparticles are non-toxic and capable of penetrating skin cells.
- These PHBV particles show promise as effective carriers for topical and transdermal drug delivery.
- Potential applications include delivery to aged/damaged skin, treatment of skin diseases like psoriasis, and gene transfer.
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