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Related Experiment Video

Updated: May 3, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Using glow stick chemistry for biological imaging.

Jen-Chieh Tseng¹, Dyane Bailey, Tanya Tupper

¹Lurie Family Imaging Center, Dana-Farber Cancer Institute, Harvard Medical School, 450 Brookline Avenue, Boston, MA, 02215, USA, jct223@gmail.com.

Molecular Imaging and Biology

|February 11, 2014

Summary

This study introduces glow stick chemistry for non-invasive imaging of oxidative stress and reactive oxygen species (ROS) in living animals, visualizing ROS production in phagocytosis and mitochondria.

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Area of Science:

Biomedical Imaging
Chemiluminescence and Photoluminescence

Background:

Phagocytes produce reactive oxygen species (ROS) to combat microorganisms.
Mitochondrial respiration generates superoxide, a source for signaling ROS.
Existing methods for ROS imaging have limitations.

Purpose of the Study:

To develop a novel imaging strategy using glow stick chemistry.
To non-invasively visualize oxidative stress and ROS production in vivo.
To demonstrate proof-of-concept in living animal models.

Main Methods:

Utilized a ROS-reacting substrate as a chemical energy donor.
Employed targeted photoluminescent dyes as chemical energy recipients.
Applied energy transfer luminescence for imaging in phagosomes and mitochondria.

Main Results:

Successfully visualized ROS production associated with phagocytosis in mice.
Demonstrated imaging of ROS from mitochondrial respiration in vivo.
Confirmed the feasibility of glow stick chemistry for ROS detection.

Conclusions:

Glow stick imaging offers a hybrid approach combining chemiluminescence and photoluminescence.
This technique enables deep tissue imaging with red or far-red emission.
Provides a new tool for studying oxidative stress and ROS-related biological processes.