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Updated: May 3, 2026

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
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BioID: a screen for protein-protein interactions.

Kyle J Roux1,2, Dae In Kim1, Brian Burke3

  • 1Children's Health Research Center, Sanford Research, North Sioux Falls, South Dakota.

Current Protocols in Protein Science
|February 11, 2014
PubMed
Summary

BioID is a proximity-dependent biotinylation method for identifying protein interactions in living cells. This technique uses a promiscuous biotin ligase to tag and isolate proteins interacting with a protein of interest.

Keywords:
BioIDbiotinylationprotein-protein interactionproximity-dependent labeling

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Identifying protein-protein interactions is crucial for understanding cellular mechanisms.
  • Existing methods often struggle with weak, transient, or insoluble interactions within a native cellular environment.

Purpose of the Study:

  • To introduce and describe the BioID (proximity-dependent biotinylation) technique.
  • To highlight BioID's capability in screening for physiologically relevant protein interactions in living cells.

Main Methods:

  • A promiscuous biotin ligase is fused to a protein of interest.
  • The fusion protein is expressed in cells, biotinylating proximal endogenous proteins.
  • Biotinylated proteins are isolated using standard biotin-affinity capture for identification.

Main Results:

  • BioID enables the identification of candidate interactors for a protein of interest.
  • The method is effective for insoluble proteins and weak or transient interactions.
  • BioID can be temporally regulated and applied across diverse cell types.

Conclusions:

  • BioID offers a powerful approach for discovering protein interactions in their native cellular context.
  • The technique's versatility makes it applicable to a wide range of biological systems.
  • BioID facilitates the study of complex protein networks within living cells.