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Updated: May 3, 2026
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
Published on: September 17, 2010
Electroporation-mediated delivery of genes in rodent models of lung contusion
David Machado-Aranda1, Krishnan Raghavendran
1Division of Acute Care Surgery, Department of Surgery, University of Michigan, Ann Arbor, MI, USA.
Abstract:
Several of the biological processes involved in the pathogenesis of acute lung injury and acute respiratory distress syndrome after lung contusion are regulated at a genetic and epigenetic level. Thus, strategies to manipulate gene expression in this context are highly desirable not only to elucidate the mechanisms involved but also to look for potential therapies. In the present chapter, we describe mouse and rat models of inducing blunt thoracic injury followed by electroporation-mediated gene delivery to the lung. Electroporation is a highly efficient and easily reproducible technique that allows circumvention of several of lung gene delivery challenges and safety issues present with other forms of lung gene therapy.
Insights
Researchers developed novel mouse and rat models for studying acute lung injury and acute respiratory distress syndrome. These models utilize electroporation for efficient gene delivery, offering potential new therapies for lung diseases.
Area of Science:
- Molecular biology
- Genetics
- Pulmonology
Background:
- Acute lung injury and acute respiratory distress syndrome pathogenesis involve genetic and epigenetic regulation.
- Targeting gene expression is crucial for understanding disease mechanisms and developing therapies.
Purpose of the Study:
- To describe established mouse and rat models for inducing blunt thoracic injury.
- To detail electroporation-mediated gene delivery to the lung in these models.
Main Methods:
- Induction of blunt thoracic injury in mouse and rat models.
- Application of electroporation for gene delivery to the lung.
- Utilizing established animal models for studying lung injury and gene therapy.
Main Results:
- Electroporation proves to be a highly efficient and reproducible gene delivery technique.
- This method effectively overcomes challenges associated with traditional lung gene therapy.
Conclusions:
- The described models and gene delivery method provide a valuable platform for investigating acute lung injury and acute respiratory distress syndrome.
- Electroporation-mediated gene delivery offers a promising strategy for potential therapeutic interventions in lung diseases.
