Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Vaccinations01:51

Vaccinations

44.1K
Overview
44.1K
Induced Electric Fields: Applications01:27

Induced Electric Fields: Applications

2.7K
An important distinction exists between the electric field induced by a changing magnetic field and the electrostatic field produced by a fixed charge distribution. Specifically, the induced electric field is nonconservative because it does not work in moving a charge over a closed path. In contrast, the electrostatic field is conservative and does no net work over a closed path. Hence, electric potential can be associated with the electrostatic field but not the induced field. The following...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic analysis of the immune response to <i>in vivo</i> gene electrotransfer in colorectal cancer.

Molecular therapy. Nucleic acids·2025
Same author

Immunogenic Effects and Clinical Applications of Electroporation-Based Treatments.

Vaccines·2024
Same author

Azoxymethane/Dextran Sodium Sulfate (AOM/DSS) Model of Colorectal Cancer.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Mouse Melanoma Model in Tumor Vaccines and Immunotherapy Research.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Label-free electrochemical immunosensor as a reliable point-of-care device for the detection of Interleukin-6 in serum samples from patients with psoriasis.

Frontiers in chemistry·2023
Same author

Evaluation of Systemic Genotoxic/Oxidative and Proinflammatory Effects in Workers of a Titanium Dioxide Production Plant.

BioMed research international·2023

Related Experiment Video

Updated: May 3, 2026

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
06:37

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

Published on: October 18, 2012

15.5K

Intramuscular DNA vaccination protocols mediated by electric fields.

Pieranna Chiarella1, Emanuela Signori

  • 1Laboratory of Molecular Pathology and Experimental Oncology, CNR-IFT, Rome, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|February 11, 2014
PubMed
Summary

Electrotransfer significantly enhances DNA vaccination, improving both humoral and cellular immune responses. This gene electrotransfer method shows promise for more effective DNA-based vaccines against infectious and cancer diseases.

More Related Videos

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
08:13

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

23.0K
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
15:56

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

Published on: October 19, 2009

17.4K

Related Experiment Videos

Last Updated: May 3, 2026

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
06:37

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

Published on: October 18, 2012

15.5K
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
08:13

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

23.0K
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
15:56

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

Published on: October 19, 2009

17.4K

Area of Science:

  • Immunology
  • Vaccinology
  • Biotechnology

Background:

  • Vaccination is a cornerstone of infectious disease prevention.
  • DNA vaccines offer a promising strategy for inducing protective immunity.
  • Current DNA vaccine strategies require enhancement due to poor immunogenicity.

Purpose of the Study:

  • To evaluate electrotransfer as a method to improve DNA-based vaccination protocols.
  • To demonstrate the effectiveness of gene electrotransfer in stimulating immune responses.
  • To describe a specific DNA vaccination procedure combined with electrotransfer and hyaluronidase pretreatment.

Main Methods:

  • Utilizing plasmid DNA for vaccination.
  • Employing gene electrotransfer to enhance DNA vaccine delivery and immunogenicity.
  • Incorporating hyaluronidase pretreatment in vaccination procedures.

Main Results:

  • Gene electrotransfer effectively stimulates both humoral and cellular immune responses.
  • Preclinical trials show successful prime-boost combination protocols using gene electrotransfer.
  • Phase I clinical trials indicate good tolerability and safety of gene electrotransfer.

Conclusions:

  • Electrotransfer is an effective strategy for improving DNA-based vaccination protocols.
  • This approach holds potential for developing enhanced vaccines against infectious and cancer diseases.
  • The described plasmid DNA vaccination with electrotransfer and hyaluronidase is a viable laboratory procedure.