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Laboratory culture and maintenance of Stenotrophomonas maltophilia

Osama Mahdi1, Bridget Eklund, Nathan Fisher

  • 1Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, North Dakota.

Current Protocols in Microbiology
|February 11, 2014
PubMed

Insights

Stenotrophomonas maltophilia, a soil bacterium, is an emerging hospital-acquired pathogen. This guide provides essential protocols for its laboratory growth and maintenance.

Area of Science:

  • Microbiology
  • Bacteriology
  • Infectious Diseases

Background:

  • Stenotrophomonas maltophilia is a ubiquitous soil bacterium.
  • It is increasingly recognized as an emerging nosocomial pathogen, causing hospital-acquired infections.

Purpose of the Study:

  • To provide standardized protocols for the in vitro growth and maintenance of Stenotrophomonas maltophilia.
  • To support research and clinical laboratory work involving this opportunistic pathogen.

Main Methods:

  • Detailed procedures for cultivating Stenotrophomonas maltophilia on various media.
  • Guidelines for stock culture preparation, storage, and revival.
  • Methods for ensuring strain purity and viability.

Main Results:

  • Established protocols enable reliable in vitro cultivation of S. maltophilia.
  • The methods ensure consistent bacterial growth for experimental use.
  • Protocols are designed for ease of implementation in standard microbiology laboratories.

Conclusions:

  • Standardized protocols are crucial for reproducible research on Stenotrophomonas maltophilia.
  • Effective laboratory maintenance is essential for studying its role as a nosocomial pathogen.
  • These protocols facilitate further investigation into S. maltophilia pathogenesis and treatment.
Keywords:
Stenotrophomonas maltophiliahospital acquired infectionlaboratory growthnosocomial

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