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Laboratory growth and maintenance of Streptococcus pyogenes (the Group A Streptococcus, GAS)

Kanika Gera1, Kevin S McIver1

  • 1Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland.

Current Protocols in Microbiology
|February 11, 2014
PubMed

Insights

Streptococcus pyogenes, a human pathogen causing millions of infections and deaths, requires specific growth conditions. This guide details methods for its cultivation and maintenance to facilitate further research.

Area of Science:

  • Microbiology
  • Bacteriology
  • Infectious Diseases

Background:

  • Streptococcus pyogenes is a significant human pathogen responsible for numerous infections and deaths globally.
  • This Gram-positive bacterium is nutritionally demanding and exhibits strict growth requirements.
  • Understanding its growth is crucial for studying its pathogenesis and developing treatments.

Purpose of the Study:

  • To provide a detailed protocol for the growth and maintenance of Streptococcus pyogenes.
  • To facilitate laboratory research on this important human pathogen.

Main Methods:

  • Describes optimal culture media and conditions for Streptococcus pyogenes.
  • Outlines procedures for long-term storage and subculturing of the bacteria.

Main Results:

  • Successfully established reliable methods for cultivating Streptococcus pyogenes.
  • Ensured consistent viability and purity of bacterial cultures for experimental use.

Conclusions:

  • The described methods enable consistent growth and maintenance of Streptococcus pyogenes.
  • These protocols are essential for advancing research into Streptococcus pyogenes infections and control.
Keywords:
Streptococcus pyogeneschemically defined mediumfacultative anaerobefirmicutethe Group A Streptococcus

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