Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A temporal phospho-acetylome atlas of human myogenesis identifies coordinated post-translational regulation.

Molecular & cellular proteomics : MCP·2026

Same author

Stratum corneum lipid remodeling as a proposed mechanism for liposomal penetration enhancement.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026

Same author

Mas-related G protein-coupled receptor type D deficiency promotes tubulointerstitial injury and fibrosis associated with proteinuria in male mice.

Physiological reports·2026

Same author

Nucleation dynamics in amyloid-beta dimerization revealed by single-molecule fingerprinting.

Cell reports. Physical science·2026

Same author

Transfer learning in DeepLC improves LC retention time prediction across substantially different modifications and setups.

Nature communications·2026

Same author

PAF15-PCNA exhaustion governs the strand-specific control of DNA replication.

Nature·2026

Search research articles

Related Experiment Video

Updated: May 3, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Insights into the cellular response triggered by silver nanoparticles using quantitative proteomics.

Thiago Verano-Braga¹, Rona Miethling-Graff, Katarzyna Wojdyla

¹Protein Research Group and §MEMPHYS Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark , Odense, DK-5230, Denmark.

|February 12, 2014

Summary

This summary is machine-generated.

Silver nanoparticle size dictates cellular response and cell entry. Smaller 20 nm silver nanoparticles induce cellular stress and SUMOylation, while larger 100 nm particles trigger indirect pathway effects.

More Related Videos

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Published on: April 13, 2016

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

Related Experiment Videos

Last Updated: May 3, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Published on: April 13, 2016

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

Area of Science:

Nanotechnology
Cellular Biology
Toxicology

Background:

Nanoparticle use in food, materials, and medicine is rising.
Understanding nanoparticle-induced cellular responses is crucial for risk assessment and therapeutic development.

Purpose of the Study:

To investigate silver nanoparticle-protein interactions and cellular responses in human LoVo cells.
To elucidate size-dependent effects of silver nanoparticles on cellular mechanisms.

Main Methods:

Mass spectrometry-based proteomics
Western blotting
Confocal laser scanning microscopy

Main Results:

100 nm silver nanoparticles induced indirect effects via PAK, MAPK, and phosphatase 2A pathways.
20 nm silver nanoparticles caused direct cellular stress, including reactive oxygen species generation and protein carbonylation.
SUMOylation proteins were upregulated by 20 nm silver nanoparticles; these particles also entered cells, unlike 100 nm particles.

Conclusions:

Nanoparticle size is a critical determinant of cellular response and cellular uptake.
Distinct molecular mechanisms are activated by different sized silver nanoparticles.
Size-dependent cellular effects necessitate tailored risk assessments and therapeutic strategies for nanoparticles.