Production of a novel N-monomethylated dideoxysugar
James B Thoden1, Hazel M Holden
1Department of Biochemistry, University of Wisconsin , Madison, Wisconsin 53706, United States.
Abstract:
The importance of unusual deoxysugars in biology has become increasingly apparent over the past decade. Some, for example, play key roles in the physiological activities of the natural products to which they are attached. Here we describe a study of TylM1, a dimethyltransferase from Streptomyces fradiae involved in the production of dTDP-mycaminose. From this investigation, the manner in which the enzyme binds its dimethylated product has been revealed. More significantly, by providing the enzyme with an alternative substrate, it was possible to produce a monomethylated product not observed in nature. This has important ramifications for the production of unique carbohydrates that may prove useful in drug design.
Related Concept Videos
Biosynthesis of Polysaccharides
Sanger Sequencing
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Biosynthesis of Nucleic Acids
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....


