Phylogenetic diversity of GB virus C at the antigenic site of E2 protein

Amino acids at position 267-298 in E2 protein of GB virus C (GBV-C) were recognized as the antigenic site, and peptides within the region were previously reported to have inhibitory effect on HIV entry. The effect of sequence variability between different types of GBV-C on the antigenic region of the E2 protein was studied by using phylogenetic analysis. Eighty-one unique sequences encompassing this region derived from all seven GBV-C genotypes were compared to each other in this study. The results showed that GBV-C E2 antigenic nucleotide sites belonging to genotype 3 clustered together regardless of synonymous or nonsynonmous sites in the region, whereas, GBV-C E2 antigenic nucleotide sites belonging to the other 6 genotypes clustered together regardless of genotypes. Despite the fact that GBV-C genotypes might confer different degree of 'protection' against HIV, the lack of clustering as a unique group based on the amino acid differences in the antigenic site among the six genotypes suggested some other genomic regions or secondary structure of E2 protein might have played a crucial role in determining the variable protection effect of GBV-C on HIV infection.