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Phospholipid transfer protein (PLTP) deficiency impaired blood-brain barrier integrity by increasing cerebrovascular

Ting Zhou1, Qihua He2, Yawei Tong1

  • 1Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.

Biochemical and Biophysical Research Communications
|February 12, 2014
PubMed

Insights

Phospholipid transfer protein (PLTP) deficiency increases blood-brain barrier (BBB) permeability and oxidative stress. PLTP may maintain BBB integrity by transferring vitamin E and reducing oxidative stress.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Phospholipid transfer protein (PLTP) is involved in lipid metabolism and oxidative stress.
  • PLTP is highly expressed in the blood-brain barrier (BBB), but its role in BBB integrity is unknown.

Purpose of the Study:

  • To investigate the role of PLTP in maintaining blood-brain barrier integrity.
  • To explore the mechanisms underlying PLTP's function in the BBB.

Main Methods:

  • In vivo multiphoton imaging and Evans blue assay were used to assess BBB permeability.
  • Expression of tight junction proteins (occludin, ZO-1, claudin-5) was analyzed.
  • Cerebrovascular oxidative stress markers (ROS, HNE, SOD activity) were measured.

Main Results:

  • PLTP-deficient mice showed increased BBB permeability and reduced expression of tight junction proteins.
  • PLTP deficiency led to increased cerebrovascular oxidative stress (ROS, HNE) and decreased antioxidant activity (SOD).
  • Vitamin E supplementation improved BBB integrity and tight junction protein expression in PLTP-deficient mice.

Conclusions:

  • PLTP plays a crucial role in maintaining blood-brain barrier integrity.
  • PLTP may exert its protective effects by facilitating vitamin E transfer and mitigating cerebrovascular oxidative stress.

Keywords:
Blood–brain barrierOxidative stressPhospholipid transfer proteinTight junction

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