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Enzyme-Linked Immunosorbent Assay01:33

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Related Experiment Video

Updated: May 3, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
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Surface-enhanced fluorescent immunoassay on 2D silver nanotriangles array.

Zhishi Li1, Zhinan Guo2, Weidong Ruan2

  • 1State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China; State Key Lab of Marine Coatings, 4 Jinhu Road, Qingdao 266071, China; Marine Chemical Research Institute, 4 Jinhu Road, Qingdao 266071, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|February 12, 2014
PubMed
Summary

This study presents a novel silver nanotriangle array for enhanced fluorescent immunoassays. The developed array significantly boosts fluorescent signal, enabling highly sensitive detection of immune complexes.

Keywords:
ArraysImmunoassaySilver nanotriangleSurface-enhanced fluorescence

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Surface-enhanced fluorescence is crucial for sensitive bioassays.
  • Developing efficient platforms for immunoassays remains a key challenge in diagnostics.

Purpose of the Study:

  • To fabricate a long-range ordered silver nanotriangle array for enhanced fluorescent immunoassay.
  • To investigate the potential of this array in improving the sensitivity and detection limits of immunoassays.

Main Methods:

  • Fabrication of silver nanotriangle arrays using a polystyrene microsphere-based Langmuir-Blodgett (LB) template method on glass slides.
  • Performing immune reactions (antigens and labeled antibodies) on the silver nanotriangle array surface.
  • Analyzing fluorescent intensity enhancement and determining the limit of detection.

Main Results:

  • A silver nanotriangle array with approximately 100 nm height was successfully constructed.
  • A 3.11-fold enhancement in fluorescent intensity of target antibodies was achieved due to plasmon resonance and enrichment effects.
  • A limit of detection of 100 pg/mL for immune complexes was established.

Conclusions:

  • The 2D silver nanotriangle array offers a sensitive and reproducible platform for fluorescent immunoassays.
  • This platform shows significant potential for applications in proteomics, drug discovery, and diagnostics.
  • The convenient fabrication and high sensitivity make it an exciting tool for bioanalytical applications.