Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Employing molecular beacons to assess in vitro transcription with single-molecule resolution.

Scientific reports·2026

Same author

Reservoir Computing-Based Glucose Sensing With an Enzymatic Reaction Network.

Angewandte Chemie (International ed. in English)·2026

Same author

Timed batch inputs unlock substantially higher yields for enzymatic cascades.

Nature chemistry·2026

Same author

Designing soft materials through synthetic morphogenesis.

Nature communications·2026

Same author

Changes in the pH-Activity Profiles of Enzymes upon Immobilization on Polyelectrolyte-Containing Hydrogels.

Chem & bio engineering·2026

Same author

Autonomous Spatiotemporal Regulation of Reversible Hydrogel Actuators by Chemical Reaction Networks.

Advanced materials (Deerfield Beach, Fla.)·2025

Search research articles

Related Experiment Video

Updated: May 3, 2026

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

Fluorescent hydrogels for studying Ca(2+)-dependent reaction-diffusion processes.

Sergey N Semenov¹, Sjoerd G J Postma, Ilia N Vialshin

¹Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands. w.huck@science.ru.nl.

Chemical Communications (Cambridge, England)

|February 12, 2014

Summary

This summary is machine-generated.

Researchers developed a new experimental platform to study calcium ion (Ca2+) diffusion with Calbindin D28k. This method advances understanding of cellular reaction-diffusion networks crucial for cell function.

More Related Videos

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

Related Experiment Videos

Last Updated: May 3, 2026

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

Area of Science:

Biochemistry
Cell Biology
Physical Chemistry

Background:

Calcium ions (Ca2+) play critical roles in cellular signaling and processes.
Understanding Ca2+ dynamics, including diffusion, is essential for cellular function.
Ca2+-binding proteins modulate Ca2+ availability and signaling.

Purpose of the Study:

To introduce a novel and convenient experimental platform for investigating Ca2+ diffusion.
To examine Ca2+ diffusion in the presence of the Ca2+-binding protein Calbindin D28k.
To provide tools for elucidating the physical chemistry of Ca2+-dependent reaction-diffusion networks.

Main Methods:

Development of a specialized experimental setup.
Utilizing Calbindin D28k as a model Ca2+-binding protein.
Quantitative analysis of Ca2+ diffusion dynamics.

Main Results:

Successfully established a convenient experimental platform for studying Ca2+ diffusion.
Demonstrated the platform's capability in the presence of Calbindin D28k.
Generated data facilitating the study of Ca2+ binding and diffusion interactions.

Conclusions:

The developed platform offers new possibilities for studying complex Ca2+ dynamics.
This research contributes to understanding the physical chemistry of cellular reaction-diffusion systems.
The findings are relevant for various biological processes involving Ca2+ signaling.