Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 3, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

11.4K

A photochemical method for determining plasma homocysteine with limited sample processing.

Lovemore Hakuna1, Jorge O Escobedo, Mark Lowry

  • 1Department of Chemistry, Portland State University, Portland, Oregon 97207, USA. strongin@pdx.edu.

Chemical Communications (Cambridge, England)
|February 12, 2014
PubMed
Summary

This study introduces a novel method for detecting total homocysteine (tHcy) in plasma using photolytic thiyl radical formation. This technique enables selective measurement of tHcy through a unique reduction mechanism involving viologens.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toxicity, Chemistry, and Public Health Relevance of Emerging Nicotine Analog Vapes, Pods, and Pouches.

Chemical research in toxicology·2026
Same author

Analysis of Novel Cannabis Products Labeled as Containing THC-JD.

Cannabis (Albuquerque, N.M.)·2026
Same author

A comparative toxicological evaluation of nicotine and its analog 6-methyl nicotine in E-cigarette aerosol utilizing a 3D in vitro human respiratory model.

Toxicology·2026
Same author

Production of 3-Cyanopyridine upon Vaping Nicotinamide and Evaluation of the Toxicological Effects of Individual and Combined Exposures via Isobologram-Based Analysis.

Chemical research in toxicology·2025
Same author

Nucleophilic Ring-Opening of <i>N</i>-Benzoylaziridine Combined with Spirolactamization of Rhodol Enables Ratiometric Fluorescence Imaging of Hydropersulfides in <i>Arabidopsis thaliana</i>.

Analytical chemistry·2025
Same author

Cannabis concentrate vaping chemistry.

Frontiers in toxicology·2025

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Radical Chemistry

Background:

  • Total homocysteine (tHcy) is a biomarker linked to various health conditions.
  • Accurate and selective detection of tHcy in biological samples is crucial for clinical diagnostics.
  • Existing methods for tHcy detection may face challenges with selectivity or complexity.

Purpose of the Study:

  • To develop a selective and efficient method for quantifying total homocysteine (tHcy) in plasma.
  • To elucidate the mechanism of photolytic thiyl radical formation for tHcy detection.
  • To establish a new analytical approach for homocysteine measurement.

Main Methods:

  • Photolytic generation of thiyl radicals from homocysteine.
  • Reduction of viologens by homocysteine-derived radicals.

More Related Videos

Biochemical Measurement of Neonatal Hypoxia
13:13

Biochemical Measurement of Neonatal Hypoxia

Published on: August 24, 2011

13.6K
High-Density Lipoprotein-Specific Phospholipid Efflux Assay
07:08

High-Density Lipoprotein-Specific Phospholipid Efflux Assay

Published on: September 30, 2025

564

Related Experiment Videos

Last Updated: May 3, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

11.4K
Biochemical Measurement of Neonatal Hypoxia
13:13

Biochemical Measurement of Neonatal Hypoxia

Published on: August 24, 2011

13.6K
High-Density Lipoprotein-Specific Phospholipid Efflux Assay
07:08

High-Density Lipoprotein-Specific Phospholipid Efflux Assay

Published on: September 30, 2025

564
  • Intramolecular hydrogen atom transfer (HAT) mechanism.
  • Plasma sample preparation involving reduction and filtering.

    • Main Results:

    • Selective detection of total homocysteine (tHcy) was achieved through photolysis.
    • The mechanism involves the α-amino carbon-centred radical of homocysteine.
    • Viologen reduction by the homocysteine radical confirmed the proposed pathway.

    Conclusions:

    • Photolytic thiyl radical formation provides a selective route for tHcy detection in plasma.
    • The described mechanism offers a sensitive and specific analytical strategy.
    • This method holds potential for improved clinical diagnostics of tHcy levels.