Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Programmable Double Peptide Nucleic Acid-Locked Nucleic Acid Molecular Switch Enables Extraction-Free Direct Zygosity Discrimination of Single Nucleotide Polymorphisms.

Analytical chemistry·2026
Same author

Epitope-Resolved Digital SERS Profiling of Structurally Dynamic Antigens via a Multi-Epitope Bispecific Antibody Framework.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Integrated Biodevice for Parallel Magnetic Isolation and Bioelectrocatalytic Detection of Circulating DNA and RNA Biomarkers in Non-Small Cell Lung Cancer Liquid Biopsy.

ACS sensors·2026
Same author

Spatially Profiling Trace Cytokine Signatures From Microscopically Derived Skin Samples to Probe Skin Disease Inflammation.

Small methods·2026
Same author

Multifactor authentication in extracellular vesicle analysis: methods and approaches to address the heterogeneity problem.

Nature methods·2026
Same author

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes.

Nature communications·2026
Same journal

Recent developments of textile-based triboelectric nanogenerators for smart sports applications.

Biosensors & bioelectronics·2026
Same journal

One-Tube RPA-CRISPR-Cas13a assay with rational design for single-molecule detection of waterborne viruses in drinking water treatment.

Biosensors & bioelectronics·2026
Same journal

AI-driven photophysics-aware design of fluorescent probes with applications in α-synuclein biosensing and inhibitor screening.

Biosensors & bioelectronics·2026
Same journal

Three-dimensional helical integration of high-density linear microelectrode arrays and their cross-tissue applications.

Biosensors & bioelectronics·2026
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

7.0K

Microdevices for detecting locus-specific DNA methylation at CpG resolution.

Kevin M Koo1, Eugene J H Wee2, Sakandar Rauf2

  • 1Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.

Biosensors & Bioelectronics
|February 12, 2014
PubMed
Summary
This summary is machine-generated.

A new electrochemical assay, methylation-specific electro-ligase chain reaction (MS-eLCR), detects DNA methylation at single CpG sites. This rapid, low-cost method shows accuracy comparable to existing technologies for diagnostics.

Keywords:
Breast cancer cell lineCpG resolutionDNAzymeElectrocatalytic reactionLigase chain reactionLocus-specific DNA methylation

More Related Videos

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

9.3K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.3K

Related Experiment Videos

Last Updated: May 3, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

7.0K
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

9.3K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.3K

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Analytical Chemistry

Background:

  • DNA methylation is crucial in gene regulation and disease.
  • Current detection methods can be complex, costly, or lack single-CpG resolution.
  • Need for simple, rapid, and inexpensive diagnostic tools for DNA methylation analysis.

Purpose of the Study:

  • To develop a microdevice-based electrochemical assay for locus-specific DNA methylation detection.
  • To achieve single CpG dinucleotide resolution using bisulfite conversion and ligase chain reaction (LCR).
  • To establish a cost-effective alternative for routine patient diagnostics.

Main Methods:

  • Utilized ligase chain reaction (LCR) to detect C to T base changes at CpG sites after bisulfite conversion.
  • Integrated a peroxidase-mimicking DNAzyme into LCR probes for electrochemical detection (eLCR).
  • Quantified methylation levels via electrocatalytic responses proportional to DNA methylation in breast cancer cell lines and serum samples.

Main Results:

  • The methylation-specific electro-ligase chain reaction (MS-eLCR) assay detected locus-specific DNA methylation with single CpG resolution.
  • The assay required minimal starting material (0.04 pM) and detected methylation changes as low as 10-15%.
  • Accuracy was comparable to fluorescence-based and Next Generation Sequencing methods, with good reproducibility (RSD=7.9%).

Conclusions:

  • MS-eLCR offers a simple, rapid, and inexpensive method for detecting locus-specific DNA methylation.
  • The assay demonstrates potential as a low-cost alternative for routine patient diagnostics.
  • This technology enables precise interrogation of DNA methylation at specific CpG sites.