Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of small-molecule HSF1 amplifiers by high content screening in protection of cells from stress induced injury.

Biochemical and biophysical research communications·2009
Same author

Nanowire transformation by size-dependent cation exchange reactions.

Nano letters·2009
Same author

Effect of haishengsu as an adjunct therapy for patients with advanced renal cell cancer: a randomized and placebo-controlled clinical trial.

Journal of alternative and complementary medicine (New York, N.Y.)·2009
Same author

Identification of inhibitors of HSF1 functional activity by high-content target-based screening.

Journal of biomolecular screening·2009
Same author

Antitumor effects of targeting hTERT lentivirus-mediated RNA interference against KB cell lines.

Oncology research·2009
Same author

Characteristics of emissive spectrum and the removal of nitric oxide in N2/02/NO plasma with argon additive.

Journal of environmental sciences (China)·2009

Related Experiment Video

Updated: May 3, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

13.2K

Subsurface fluorescence molecular tomography with prior information.

Wei He, Huangsheng Pu, Guanglei Zhang

    Applied Optics
    |February 12, 2014
    PubMed
    Summary

    Coupling subsurface fluorescence molecular tomography (FMT) with x-ray computed tomography (XCT) improves fluorophore imaging. This hybrid approach enhances target localization and demarcation in biological tissues.

    More Related Videos

    Hybrid µCT-FMT imaging and image analysis
    13:45

    Hybrid µCT-FMT imaging and image analysis

    Published on: June 4, 2015

    14.6K
    Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts
    07:52

    Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts

    Published on: April 26, 2018

    8.6K

    Related Experiment Videos

    Last Updated: May 3, 2026

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
    12:24

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

    Published on: July 17, 2012

    13.2K
    Hybrid µCT-FMT imaging and image analysis
    13:45

    Hybrid µCT-FMT imaging and image analysis

    Published on: June 4, 2015

    14.6K
    Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts
    07:52

    Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts

    Published on: April 26, 2018

    8.6K

    Area of Science:

    • Biomedical Imaging
    • Optical Imaging
    • Medical Physics

    Background:

    • Subsurface fluorescence molecular tomography (FMT) is an emerging technique for determining fluorescence distribution in biological tissues.
    • Stand-alone FMT faces limitations in accurately reflecting fluorophore distributions due to photon diffusion and source-detector geometry.

    Purpose of the Study:

    • To enhance the performance of subsurface FMT for more accurate fluorophore distribution imaging.
    • To overcome the limitations of stand-alone FMT in biological tissue imaging.

    Main Methods:

    • Developed a hybrid imaging method coupling subsurface FMT with x-ray computed tomography (XCT).
    • Utilized XCT-derived tissue structural information to generate a Laplacian-type regularization matrix.
    • Employed the regularization matrix to guide the reconstruction of fluorophore distribution in FMT.

    Main Results:

    • The proposed hybrid method significantly improved the localization of fluorescent targets compared to conventional FMT.
    • Enhanced demarcation of fluorescent targets was achieved using structural prior information from XCT.
    • Simulation and phantom studies validated the superior performance of the coupled imaging approach.

    Conclusions:

    • Coupling XCT with subsurface FMT offers a significant advancement in fluorescence molecular imaging.
    • The integration of structural priors from XCT enhances the accuracy and resolution of FMT reconstructions.
    • This hybrid modality holds promise for more precise in vivo molecular imaging applications.