Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

907
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
907

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitotic Cdc42 waves encode PI(3,4)P<sub>2</sub> signaling and Golgi morphological state to control spindle scaling.

Science advances·2026
Same author

Implementation of an adaptive-optics assisted isoSTED nanoscope.

Nature protocols·2026
Same author

Separation estimation of two freely rotating dipole emitters near the quantum limit.

Physical review. A·2026
Same author

Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells.

bioRxiv : the preprint server for biology·2026
Same author

pan-ASLM: Axially Swept Light Sheet Microscopy for Fast and High-Resolution Imaging of Expanded Samples.

Npj imaging·2026
Same author

Tetraspanin CD82 shapes EGFR signaling outcomes through nanoscale receptor organization.

The Journal of cell biology·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

9.7K

Three dimensional single molecule localization using a phase retrieved pupil function.

Sheng Liu, Emil B Kromann, Wesley D Krueger

    Optics Express
    |February 12, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Accurate 3D superresolution microscopy relies on precise localization of fluorophores. This study introduces phase-retrieved pupil functions to correct optical aberrations, significantly improving 3D localization accuracy in biological imaging.

    More Related Videos

    Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
    07:53

    Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

    Published on: January 16, 2018

    7.5K
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    10.0K

    Related Experiment Videos

    Last Updated: May 3, 2026

    3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
    11:28

    3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

    Published on: October 1, 2014

    9.7K
    Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
    07:53

    Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

    Published on: January 16, 2018

    7.5K
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    10.0K

    Area of Science:

    • Optical microscopy
    • Biophysics
    • Superresolution imaging

    Background:

    • Localization microscopy requires fitting single-molecule intensity patterns to the microscope's point spread function (PSF).
    • Inaccurate 3D localizations occur when PSF models fail to correct for system-specific optical aberrations.

    Purpose of the Study:

    • To develop a more accurate PSF model for 3D superresolution imaging.
    • To improve the precision of 3D localization by accounting for optical aberrations.

    Main Methods:

    • Utilizing phase-retrieved pupil functions to model system aberrations.
    • Generating accurate PSFs by incorporating pupil function information for arbitrary defocus.
    • Modifying pupil functions to include depth-dependent aberrations.
    • Implementing a fast, GPU-accelerated fitting algorithm.

    Main Results:

    • Demonstrated superior localization accuracy using pupil function-generated PSFs.
    • Validated the method with dual focal plane 3D superresolution imaging of biological samples.
    • Phase retrieval effectively captures system-specific aberrations for improved PSF modeling.

    Conclusions:

    • Phase-retrieved pupil functions enable more accurate PSF generation for 3D superresolution microscopy.
    • This approach significantly enhances 3D localization precision by correcting optical aberrations.
    • The developed method offers a robust solution for high-accuracy 3D biological imaging.