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Expression of the protooncogenes in psoriatic lesions
V N Mordovtsev1, I V Starkov, E R Zabarovsky
1Central Research Institute of Dermatology and Venereology, Ministry of Public Health, Moscow, USSR.
Abstract:
The expression of cellular protooncogenes in psoriatic lesions was studied. RNA isolated from involved skin of psoriatic patients was subjected to dot-blot hybridization with cloned viral Ki-ras, myc, abl, fos, src, erbB, mos, sis, fes, and yes oncogenes. RNA isolated from the aortal epithelium, peripheral blood lymphocytes, and skin of the nonpsoriatic patients was used as control. The results indicate that the expression of Ki-ras, myc, fos, and abl genes is elevated in psoriatic lesions. The data of this work suggest that the activity of cellular oncogenes may play an important role in the development of psoriasis.
Insights
Cellular protooncogene expression, including Ki-ras, myc, fos, and abl, is elevated in psoriatic lesions. This suggests cellular oncogene activity may be crucial in psoriasis development.
Area of Science:
- Dermatology
- Molecular Biology
- Oncology
Background:
- Psoriasis is a chronic inflammatory skin condition.
- The role of cellular protooncogenes in psoriasis pathogenesis is not fully understood.
Purpose of the Study:
- To investigate the expression levels of various cellular protooncogenes in psoriatic skin lesions.
- To determine if altered oncogene expression correlates with psoriasis development.
Main Methods:
- RNA was isolated from psoriatic skin lesions and control tissues (aortal epithelium, lymphocytes, non-psoriatic skin).
- Dot-blot hybridization was performed using cloned viral oncogenes (Ki-ras, myc, abl, fos, src, erbB, mos, sis, fes, yes).
Main Results:
- Expression of Ki-ras, myc, fos, and abl genes was significantly elevated in psoriatic lesions compared to controls.
- No significant changes were noted for src, erbB, mos, sis, fes, and yes oncogenes.
Conclusions:
- The findings indicate an upregulation of specific cellular protooncogenes in psoriasis.
- Cellular oncogene activity is implicated as a potential contributing factor in the development of psoriasis.