Related Experiment Video
Updated: May 3, 2026
09:02
Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
Published on: December 10, 2015
6.8K
The SUN protein Mps3 controls Ndc1 distribution and function on the nuclear membrane
Jingjing Chen1, Christine J Smoyer, Brian D Slaughter
1Stowers Institute for Medical Research, Kansas City, MO 64110.
The Journal of Cell Biology
|February 12, 2014
Summary
Ndc1 protein interacts with Mps3 at the nuclear envelope, crucial for spindle pole body duplication in yeast. This interaction
Area of Science:
- Cell biology
- Molecular genetics
- Biochemistry
Background:
- Nuclear pore complexes (NPCs) and spindle pole bodies (SPBs) assemble within the nuclear envelope (NE) during closed mitosis.
- Ndc1 is a conserved integral membrane protein essential for the insertion of both NPCs and SPBs.
Purpose of the Study:
- To investigate the interaction between Ndc1 and Mps3 at the NE.
- To elucidate the functional significance of the Ndc1-Mps3 binding in yeast cell division.
Main Methods:
- Fluorescence cross-correlation spectroscopy (FCCS) to detect Ndc1-Mps3 interaction in live yeast cells.
- Genetic and molecular analysis of novel ndc1 alleles.
- Analysis of SPB duplication and NPC assembly.
Main Results:
- Ndc1 directly interacts with the SUN domain protein Mps3 at the NE.
- A specific ndc1 allele (ndc1-L562S) disrupts Ndc1-Mps3 binding and causes lethal SPB duplication defects.
- Deletion of POM152 suppresses the growth and Mps3 binding defects of ndc1-L562S, suggesting a role in Ndc1 distribution.
Conclusions:
- The Ndc1-Mps3 interaction is vital for proper SPB duplication.
- Ndc1's localization and function are regulated by its interaction with Mps3, influencing its distribution between NPC and SPB.
- This interaction provides a critical control point for nuclear envelope-associated complex assembly.
More Related Videos
Related Concept Videos
01:45Regulation of Nuclear Protein Sorting
2.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K
01:46Nuclear Localization Signals and Import
6.2K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
6.2K
01:34Nuclear Protein Sorting
4.8K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.8K
01:42Nuclear Export
3.7K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.7K
01:42Directionality of Nuclear Transport
3.9K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.9K
02:31Nuclear Export of mRNA
7.1K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.1K