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Determination of antibody structures

Robyn L Stanfield1

  • 1Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2014
PubMed

While antibodies share a conserved structural framework, their complementarity-determining region loops are highly variable in size and sequence. Even more variable are the potential ways these loops can be used to interact with antigen. Thus, X-ray crystal structures of antibody Fab fragments and Fab-antigen complexes are critical for a detailed understanding of the antibody-antigen recognition process. This chapter describes the basic procedures necessary for the crystallization and structure determination of antibody Fab fragments by X-ray crystallography.

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