Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transgenerational effects of bisphenol S exposure on the development of experimental asthma.

Journal of environmental science and health. Part C, Toxicology and carcinogenesis·2025

Same author

Maternal exposure to bisphenol A has transgenerational effects on the development of experimental asthma through bromodomain-containing protein 4-zinc finger DHHC-type containing 1-stimulators of interferon genes axis.

International journal of environmental health research·2025

Same author

Peptide immunotherapy for aeroallergens.

Allergy and asthma proceedings·2023

Same author

Environmental estrogen exposures alter molecular signaling in immune cells that promote the development of childhood asthma.

Molecular immunology·2023

Same author

Serum thymus and activation-regulated chemokine (TARC) levels correlate with atopic dermatitis disease severity in patients < 6 months.

Allergy and asthma proceedings·2022

Same author

Genotypic and phenotypic variability of 22q11.2 microdeletions - an institutional experience.

AIMS molecular science·2021

Search research articles

Related Experiment Video

Updated: May 3, 2026

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

Epitope mapping with random phage display library.

Terumi Midoro-Horiuti¹, Randall M Goldblum

¹Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 12, 2014

Summary

Random phage display libraries map epitopes, including conformational and linear types. This protocol details using a commercial peptide phage display library for reliable epitope mapping.

Area of Science:

Biochemistry
Molecular Biology
Immunology

Background:

Epitope mapping is crucial for understanding antibody-antigen interactions.
Phage display technology offers a powerful tool for identifying epitopes.
Conformational and linear epitopes require distinct mapping strategies.

Purpose of the Study:

To provide a protocol for epitope mapping using random phage display libraries.
To detail the experience with a commercially available peptide phage display library.
To demonstrate the effectiveness of this method for identifying both linear and conformational epitopes.

Main Methods:

Utilizing random peptide phage display libraries of varying lengths and with circularization options.
Implementing a standardized protocol for screening and selection of phage clones.

More Related Videos

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Related Experiment Videos

Last Updated: May 3, 2026

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Characterizing selected peptides for their ability to bind target antigens.

Main Results:

Successful identification of both linear and conformational epitopes using the described protocol.
Demonstration of good results with a commercially available peptide phage display library.
The protocol is adaptable for different epitope mapping applications.

Conclusions:

Random phage display is a versatile technique for comprehensive epitope mapping.
The presented protocol offers a reliable and effective method for researchers.
Commercial peptide phage display libraries provide a practical approach to epitope discovery.