Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme activity gel analysis of human immunodeficiency virus reverse transcriptase.

M C Starnes1, W Y Gao, R Y Ting

  • 1Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill 27514.

The Journal of Biological Chemistry
|April 15, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Effect of accurately localized mini anterolateral thigh perforator flap in repairing medium-sized skin and soft tissue defects in fingers].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2024
Same author

Exploring the Effectiveness of the Introduction of Automated Physiological Monitoring Devices.

Studies in health technology and informatics·2021
Same author

[Analysis on antimicrobial sensitivity of <i>Neisseria meningitidis</i> in China from 2005 to 2019].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2021
Same author

Study of expiratory droplet dispersion and transport using a new Eulerian modeling approach.

Atmospheric environment (Oxford, England : 1994)·2020
Same author

[Molecular characteristics of serogroup B <i>neisseria meningitidis</i>, China].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2019
Same author

[Investigation of the cognition and behavior on drug safety in Beijing middle school students].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2017
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
See all related articles

The p66 component of human immunodeficiency virus (HIV) reverse transcriptase is catalytically active, responsible for DNA synthesis. The p51 component showed no DNA synthetic activity in this study.

Area of Science:

  • Biochemistry
  • Virology
  • Molecular Biology

Background:

  • Human immunodeficiency virus (HIV) reverse transcriptase is a key enzyme for viral replication.
  • Understanding the specific roles of its subunits is crucial for developing antiviral therapies.

Purpose of the Study:

  • To identify the catalytically active component of HIV reverse transcriptase.
  • To differentiate the enzymatic functions of the p66 and p51 subunits.

Main Methods:

  • Activity gel analysis of purified HIV reverse transcriptase and infected cell extracts.
  • Denaturing polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS) removal.
  • Assays for DNA and RNA-directed DNA synthesis.

Main Results:

Related Experiment Videos

  • Purified HIV reverse transcriptase consists of approximately equal proportions of p66 and p51 proteins.
  • The p66 component was sufficient for both DNA- and RNA-directed DNA synthesis.
  • No DNA synthetic activity was detected for the p51 component.

Conclusions:

  • The p66 subunit of HIV reverse transcriptase possesses the catalytic activity for DNA synthesis.
  • The p51 subunit does not appear to contribute to the DNA synthetic function of the enzyme.
  • No other HIV-specific DNA polymerases were identified in infected cell extracts besides p66.