Related Experiment Video
Updated: May 3, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
2PE-STED microscopy with a single Ti:sapphire laser for reduced illumination
Qifeng Li1, Yang Wang2, Da Chen1
1College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, China ; Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin, China.
Abstract:
We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles.
More Related Videos
12:54Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
Published on: July 17, 2016
09:06In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
Published on: December 20, 2021
Related Concept Videos
Confocal Fluorescence Microscopy
Super-resolution Fluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy