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Updated: May 3, 2026

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
Published on: April 9, 2014
[Application of quantitative evaluation on multicomponent drug interaction in anti-HIV research]
Pei-Zhi Dong1, Yong-Xiang Gao1, Xian-Bo Wu1
1College of Basic Medicine, Chengdu University of TCM, Chengdu 610075, China.
Objective:
To quantitatively evaluate mutual relations of 4 component drugs in anti-HIV action.
Methods:
The effect of TCM four components on cell growth was detected using MTT assay. The antiviral effects of 4 components were observed at the maximal nonvenomous dose. The combination index (CI) value of combined two or four components were calculated using median-effect principle. The mutual relations of two or four components for antiviral actions were assessed using CI.
Results:
Synergism was dominant in combination of A and B, and the effect was dose-dependent. Antagonism was dominant in combination of C and D, and the effect was dose-dependent. But the combination of A, B, C, and D was synergistic when the inhibition rate was over 10%.
Conclusion:
Median-effect principle can be used to quantitatively assess the anti-HIV effect of four components.
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