Sodium and potassium currents involved in action potential propagation in normal bovine lactotrophs

1. The properties of whole-cell and single-channel Na+ and K+ currents in immunocytochemically identified bovine lactotrophs were studied using the patch-clamp technique. 2. In the whole-cell, current-clamp mode, cells had membrane potentials of -94.7 +/- 6.7 mV and input resistances of 2-17 G omega. Current-induced action potentials were recorded with a threshold around -35 mV and amplitude of 40-65 mV. Repetitive firing was not sustained at frequencies greater than 1-2 Hz without total inactivation. 3. Under voltage clamp, action potentials were shown to be composed of an inward TTX-sensitive Na+ current and an outward K+ current that was abolished by internal Cs+. 4. The isolated Na+ current had a threshold for activation around -35 mV and rapidly inactivated to a steady state during a test voltage pulse. Inactivation was strongly voltage-dependent, with the Na+ current being half-inactivated at -20 mV. 5. Recovery from inactivation was voltage dependent and at a holding potential of -60 mV, 50% reactivation was achieved after 420 ms. The implications of this long reactivation time on sustained action potential frequency are discussed. 6. Single Na+ channel activity was examined with the outside-out patch configuration and yielded single-channel conductances of 22.5 pS. Reconstruction of the voltage and time dependence of single-channel currents provided an accurate picture of the whole-cell Na+ current. 7. Whole-cell outward current carried by K+ in the absence of Na+ and Ca2+ had a large conductance, was slowly activated and demonstrated no inactivation. A second, more rapidly activating Ca2+-dependent K+ current could also be demonstrated. 8. Ensemble analysis of whole-cell K+ currents in the absence of Ca2+ showed underlying single-channel amplitudes of 1.2 pA at +10 mV, with the lactotroph having about 350 active channels at this potential. 9. Recordings of single K+ channels also demonstrated two classes of channel: a small (50 pS) voltage-activated channel and a higher-conductance (100 pS) Ca2+- sensitive channel. 10. Prolactin secretion was shown to be TTX-insensitive but sensitive to membrane potential, demonstrated as increased release following increased external K+ but not Na+ concentration.(ABSTRACT TRUNCATED AT 400 WORDS)