Statistical analysis of channel current from a membrane patch. II. A stochastic theory of a multi-channel system in...

A general stochastic theory is presented for analysis of current records of a patch containing an arbitrary number (N) of independent homologous channels in the steady-state. We give the "basic theorem" that at the instant of any open (or shut) transition of a channel, the other N-1 channels are located in each state with a probability equal to those in the steady-state, if enough transitions are observed. Using the "basic theorem", we derived: (a) the time-dependent open and shut frequencies after a definite type of transition, and (b) the probability density functions (pdf) of the duration of any period between two successive transitions. Briefly, the main results obtained were: (1) The time-dependent open (or shut) transition frequency after every shut (or open) transition at t = 0 in an N-channel patch, fJSh,Op(t)(N) (or fJOp,Sh(t)(N)), is the same as that of a one-channel patch except for the value of the constant. (2) In the all-shut (or all-open) period of a patch, the average duration of the period is 1/N, and the slowest exponential decay constant contained in the pdf is N times those of a single channel patch, respectively. (3) An example calculation for small N showed that the stochastic properties of a single channel can be obtained even when N is uncertain, if the channel open probability is small and exponential decay constants are separated. (4) When the channels are in equilibrium, the pdf of duration of every type of period in the patch is described by a sum of exponential terms with positive coefficients. This also holds for fJSh,Op(t)(N) and fJOp,Sh(t)(N).