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Nuclear protein with sequence homology to translation initiation factor eIF-4A
Nature
|April 21, 1988
Summary
The p68 protein, a nuclear antigen crucial for cell growth and division, was identified through its cross-reaction with SV40 large T antigen. Its homology to translation factor eIF-4A suggests a role as a DNA or RNA helicase.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- p68 protein is a conserved nuclear antigen.
- It is found in dividing cells but not quiescent cells.
- p68 is induced by serum stimulation in fibroblasts.
Purpose of the Study:
- To investigate the function and identity of the p68 protein.
- To understand the molecular basis of its cross-reaction with SV40 large T antigen.
- To explore its potential role in cell growth regulation.
Main Methods:
- Immunological detection using anti-SV40 large T monoclonal antibody PAb204.
- Sequencing of human complementary DNA (cDNA) encoding p68.
- Sequence homology analysis with known proteins.
Main Results:
- p68 exhibits a granular nuclear distribution and is induced by serum.
- Sequencing revealed homology between p68 and the translation initiation factor eIF-4A.
- Homology suggests p68 may function as an ATP-dependent DNA or RNA helicase.
Conclusions:
- p68 protein is a growth-regulated nuclear antigen.
- Its homology to eIF-4A and T antigen suggests helicase activity.
- p68 likely plays a role in DNA/RNA processing, replication, or transcription, essential for cell growth.