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Effect of glucans from Caripia montagnei mushroom on TNBS-induced colitis
Marilia da S Nascimento Santos1, Joedyson Emmanuel de M Magalhães2, Luiza Sheyla Evenni P Will Castro3
1Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Salgado Filho, 3000, Bairro L. Nova, CEP 59078-970, Natal, RN, Brazil. mariliabio84@gmail.com.
Abstract:
In this study, we evaluated the effect of different doses of polysaccharides extracted from Caripia montagnei mushroom at different intervals of treatment on colonic injury in the model of colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The FT-IR analysis and NMR showed that the polysaccharides from this species of mushroom are composed of α- and β-glucans. The colonic damage was evaluated by macroscopic, histological, biochemical and immunologic analyses. The results showed the reduction of colonic lesions in all groups treated with the glucans. Such glucans significantly reduced the levels of IL-6 (50 and 75 mg/kg, p < 0.05), a major inflammatory cytokine. Biochemical analyses showed that the glucans from C. montagnei acted on reducing levels of alkaline phosphatase (75 mg/kg, p < 0.01) and myeloperoxidase (p < 0.001), a result confirmed by the reduction of cellular infiltration observed microscopically. The increase of catalase activity possibly indicates a protective effect of these glucans on colonic tissue, confirming their anti-inflammatory potential.
Insights
Polysaccharides from Caripia montagnei mushroom, composed of alpha- and beta-glucans, effectively reduced colonic injury and inflammation in a colitis model. These mushroom glucans demonstrated significant anti-inflammatory potential by lowering key cytokine and enzyme levels.
Area of Science:
- Mycology
- Immunology
- Pharmacology
Background:
- Colitis, induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS), is a significant inflammatory condition.
- Mushroom-derived polysaccharides are recognized for their immunomodulatory and therapeutic properties.
Purpose of the Study:
- To evaluate the therapeutic potential of polysaccharides from Caripia montagnei mushroom in a TNBS-induced colitis model.
- To investigate the anti-inflammatory effects of C. montagnei glucans on colonic injury.
Main Methods:
- Extraction and characterization (FT-IR, NMR) of polysaccharides from Caripia montagnei.
- Induction of colitis in a model using 2,4,6-trinitrobenzene sulfonic acid (TNBS).
- Assessment of colonic damage via macroscopic, histological, biochemical, and immunological analyses.
Main Results:
- Caripia montagnei polysaccharides, identified as alpha- and beta-glucans, significantly reduced colonic lesions.
- Treatment with glucans led to a significant decrease in the inflammatory cytokine IL-6.
- Reduced levels of alkaline phosphatase and myeloperoxidase, along with decreased cellular infiltration, were observed.
Conclusions:
- Polysaccharides from Caripia montagnei exhibit significant anti-inflammatory and protective effects in experimental colitis.
- The observed increase in catalase activity suggests a protective role for these glucans in colonic tissue.
- C. montagnei glucans represent a promising natural therapeutic agent for inflammatory bowel diseases.
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