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Related Experiment Video

Updated: May 3, 2026

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

Dissecting mammalian immunity through mutation.

¹Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.

Immunology and Cell Biology

|February 13, 2014

Summary

Single-gene mutations can impair the immune system, offering insights into human immunology. Advanced methods like CRISPR/Cas9 mutagenesis are crucial for understanding gene function in immunity.

Area of Science:

Immunology
Genetics
Molecular Biology

Background:

The immune system evolved through mutation and natural selection.
Increasingly, single-gene mutations are identified that compromise immune function.
Understanding these mutations is key to advancing human immunology.

Purpose of the Study:

To review the discovery and creation of mutations impacting mammalian immunity.
To highlight the utility of mutagenesis in defining gene function.
To emphasize genome-wide approaches for systematic gene function analysis.

Main Methods:

Review of existing literature on mutation discovery in immunity.
Focus on chemical and CRISPR/Cas9 mutagenesis techniques.
Discussion of methods for measuring physiological effects of mutations.

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Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

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Published on: November 1, 2011

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Related Experiment Videos

Last Updated: May 3, 2026

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

Published on: November 1, 2011

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Main Results:

Single-gene mutations provide valuable models for studying immune deficiencies.
Genome-wide mutagenesis enables systematic functional genomics.
CRISPR/Cas9 and chemical mutagenesis are powerful tools for immune gene discovery.

Conclusions:

Mutations are instrumental in dissecting the complexities of the immune system.
Modern mutagenesis techniques accelerate the understanding of immune gene function.
Further research using these tools will enhance our knowledge of human immunology.