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Antibody-based proteomics and biomarker research - current status and limitations
1Translational Technologies and Bioinformatics, Pharma Research and Early Development, F. Hoffmann-La Roche AG, Basel, Switzerland.
Proteomics
|February 13, 2014
Summary
Antibody-based proteomics enhance biomarker discovery and validation, especially in complex samples like body fluids. Combining antibodies with mass spectrometry (MS) overcomes MS sensitivity limitations for high-throughput marker evaluation.
Area of Science:
- Biomarker Discovery
- Proteomics
- Analytical Chemistry
Background:
- Proteomics is crucial for biomarker discovery and validation.
- Mass spectrometry (MS) is a primary tool for hypothesis-free biomarker discovery.
- MS has limited sensitivity in complex samples, particularly body fluids with wide protein dynamic ranges.
Purpose of the Study:
- To review the benefits and limitations of antibody-based proteomics.
- To assess antibody-based proteomics in preclinical and clinical biomarker research.
- To explore applications in body fluid and tissue biomarker discovery and validation.
Main Methods:
- Review of antibody-based proteomics techniques.
- Analysis of mass spectrometry (MS) in proteomics.
- Evaluation of antibody-based methods for sensitivity enhancement.
Main Results:
- Antibody-based technologies offer higher sensitivity compared to MS alone.
- Antibody-based proteomics effectively addresses challenges in complex samples.
- The integration of antibodies and MS presents a powerful approach for biomarker research.
Conclusions:
- Antibody-based proteomics is vital for high-throughput biomarker evaluation.
- Combining antibodies with MS leverages the strengths of both techniques.
- This integrated approach opens new possibilities in biomarker discovery and validation.
