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Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry
Published on: January 29, 2016
Characterization of typical taste and odor compounds formed by Microcystis aeruginosa
Kejia Zhang1, Tsair Fuh Lin2, Tuqiao Zhang3
1College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, China. zhangkj@zju.edu.cn
Abstract:
Production and characteristics of typical taste and odor (T&O) compounds by Microcystis aeruginosa were investigated. A few terpenoid chemicals, including 2-MIB, beta-cyclocitral, and beta-ionone, and a few sulfur compounds, such as dimethyl sulfide and dimethyl trisulfide, were detected. beta-Cyclocitral and beta-carotene concentrations were observed to be relevant to the growth phases of Microcystis. During the stable growth phase, 41-865 fg/cell of beta-cyclocitral were found in the laboratory culture. beta-Cyclocitral concentrations correlated closely with beta-carotene concentrations, with the correlation coefficient R2 = 0.96, as it is formed from the cleavage reaction of beta-carotene. For dead cell cases, a high concentration of dimethyl trisulfide was detected at 3.48-6.37 fg/cell. Four T&O compounds, including beta-cyclocitral, beta-ionone, heptanal and dimethyl trisulfide, were tested and found to be able to inhibit and damage Microcystis cells to varying degrees. Among these chemicals, beta-cyclocitral has the strongest ability to quickly rupture cells.
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