Related Experiment Video
Updated: May 3, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
Published on: June 12, 2016
Moment analysis of chromatographic behavior of separation media for fast HPLC
1Department of Chemistry, Faculty of Science, Rikkyo University.
Abstract:
The van Deemter equation and moment equations were used for analyzing the flow rate dependence of HETP experimentally measured in order to clarify chromatographic behavior from the kinetic points of view in columns packed with C18-silica monolith and C18-core-shell particles under RPLC conditions. They provided some information about molecular diffusion, eddy diffusion, and mass transfer kinetics in the columns. Additionally, the value of intra-stationary phase diffusivity was determined in the range of 10(-7)-10(-6) cm(2) s(-1) by the moment analysis. Information about the contribution of the mass transfer resistance in the external liquid film around the stationary phase to HETP was also obtained. Its contribution increases with an increase in the flow velocity of the mobile phase. It was concluded that the moment equations can provide more detailed information about the kinetic behavior in the columns than the van Deemter equation.
More Related Videos
10:21Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
11:25Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
Ion-Exchange Chromatography
Chromatographic Methods: Terminology
High-Performance Liquid Chromatography: Types of Detectors