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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Phototransformable fluorescent proteins: which one for which application?

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  • 1Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, F-38000, Grenoble, France, virgile.adam@ibs.fr.

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Phototransformable fluorescent proteins (PTFPs) revolutionize cell biology imaging. These advanced tools enable unprecedented studies of molecular trafficking, single-cell tracking, and live-cell single-molecule observation.

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biotechnology

Background:

  • Fluorescent proteins (FPs) are essential, minimally invasive tools for live-cell imaging.
  • Genetically encoded FPs can be fused to proteins of interest, enabling specific labeling.
  • Recent advancements in phototransformable fluorescent proteins (PTFPs) have expanded imaging capabilities.

Purpose of the Study:

  • To review recent developments in PTFPs.
  • To highlight PTFP contributions to studies in cells, tissues, and organisms.
  • To discuss specific applications and microscopy technique complementarities.

Main Methods:

  • Review of recent literature on PTFPs.
  • Focus on applications in optical imaging and live-cell studies.
  • Discussion of PTFP integration with microscopy techniques.

Main Results:

  • PTFPs enable advanced imaging techniques like single-molecule localization microscopy.
  • PTFPs facilitate intracellular tracking of photoactivated molecules.
  • PTFPs are crucial for monitoring trafficking, individualizing cells, and observing single molecules.

Conclusions:

  • PTFPs represent a significant leap in optical imaging capabilities for biological research.
  • Continued engineering of PTFPs promises more powerful and versatile imaging tools.
  • PTFPs are vital for progress in cell biology, optobiology, and biotechnology.