Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in heparan sulfate correlate with increased glomerular permeability.

G C Groggel1, J Stevenson, P Hovingh

  • 1Department of Medicine, University of Utah School of Medicine, Salt Lake City.

Kidney International
|February 1, 1988
PubMed
Summary

Experimental membranous nephropathy in rabbits impairs glomerular filtration. This study found that the disease damages both the size and charge barriers, linked to changes in heparan sulfate structure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes Following Pedestal Cup Reconstruction of (Impending) Pathological Fractures of the Acetabulum due to Metastatic Bone Disease.

Indian journal of surgical oncology·2024
Same author

Current updates in MSK infection imaging: A narrative review.

Journal of clinical orthopaedics and trauma·2024
Same author

Correction to: Test Yourself question: Incidental hip lesion on PET‑CT.

Skeletal radiology·2023
Same author

Test yourself answer: incidental hip lesion on PET-CT.

Skeletal radiology·2023
Same author

Test Yourself question: Incidental hip lesion on PET-CT.

Skeletal radiology·2023
Same author

Mixed-reality technology for clinical communication: objective assessment of the HoloLens 2 as a clinical communication device in a simulated on-call scenario.

The Journal of laryngology and otology·2023

Area of Science:

  • Nephrology
  • Biochemistry
  • Physiology

Background:

  • The glomerular capillary wall is a critical barrier regulating kidney filtration.
  • Heparan sulfate (HS) is essential for the charge-selective function of this barrier, preventing polyanion filtration.
  • Membranous nephropathy (MN) is a kidney disease characterized by glomerular damage.

Purpose of the Study:

  • To investigate alterations in charge and size selectivity of the glomerular filtration barrier in a rabbit model of experimental membranous nephropathy.
  • To characterize biochemical changes in glomerular heparan sulfate in this model.

Main Methods:

  • Experimental membranous nephropathy was induced in rabbits using cationic bovine serum albumin injections.
  • Fractional clearance of charged and uncharged dextrans relative to inulin was measured.

Related Experiment Videos

  • Sulfate incorporation into glomerular glycosaminoglycans was assessed, and glomerular heparan sulfate was isolated and characterized.

    • Main Results:

    • Animals with membranous nephropathy exhibited significant proteinuria.
    • While total sulfate incorporation remained unchanged, the proportion of [35S] incorporated into heparan sulfate decreased, indicating altered HS synthesis or composition.
    • Heparan sulfate from MN kidneys showed reduced charge density, and fractional clearance of neutral and charged dextrans increased, indicating impaired size and charge selectivity.

    Conclusions:

    • Experimental membranous nephropathy leads to a loss of both charge and size selectivity in the glomerular filtration barrier.
    • These functional deficits correlate with structural changes in glomerular heparan sulfate, specifically a reduction in its negative charge.
    • This suggests that alterations in heparan sulfate are a key mechanism underlying glomerular dysfunction in membranous nephropathy.