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Serotype determination of adenoviruses in children with respiratory infection
Yusuf Ghasemi1, Manoochehr Makvandi, Ali Reza Samarbafzadeh
1Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Insights
Adenovirus serotypes were identified in children with acute respiratory infections in Iran. Adenovirus type 5 (Ad5) was the predominant type, indicating its significance in pediatric respiratory illness.
Area of Science:
- Virology
- Pediatrics
- Infectious Diseases
Background:
- Acute respiratory infections (ARI) pose a significant health burden in children globally.
- Adenoviruses are a common cause of viral respiratory infections in pediatric populations.
- Understanding the prevalent adenovirus serotypes is crucial for effective disease management and prevention.
Purpose of the Study:
- To identify and characterize adenovirus serotypes causing ARI in children.
- To determine the epidemiological distribution of adenovirus serotypes in Khoozestan province, Iran.
- To establish the role of specific adenovirus serotypes in pediatric respiratory infections.
Main Methods:
- Collection of 163 nasopharyngeal swabs from children (1-15 years) hospitalized with ARI.
- Detection of adenoviruses using nested polymerase chain reaction (PCR).
- Sequencing of positive PCR products to confirm adenovirus serotypes.
Main Results:
- Adenoviruses were detected in 18.4% (30/163) of the samples.
- Sequencing identified Adenovirus type 5 (Ad5) and Adenovirus type 2 (Ad2).
- Ad5 accounted for 86.3% (19/22) and Ad2 for 13.7% (3/22) of the identified serotypes.
Conclusions:
- Adenoviruses, particularly Ad5, are a significant etiological agent of respiratory tract infections in children in the study region.
- The findings highlight the importance of Ad5 in pediatric ARI in Khoozestan, Iran.
- Further research on adenovirus epidemiology can inform public health strategies.
Objective:
To determine adenovirus serotypes among children with acute respiratory infection (ARI) in Khoozestan province, Iran during years 2010-2011.
Methods:
One hundred sixty three nasopharyngeal swabs were collected from children between 1 and 15 y who were hospitalized for the acute respiratory infection. The viral DNA was extracted from the nasopharyngeal swabs and adenoviruses were detected by Nested PCR. Positive PCR samples were sequenced in order to confirm the adenovirus serotypes.
Results:
Out of 163 samples, 30 (18.4 %) were positive for adenoviruses by nested PCR. Twenty two PCR products were sequenced and recognized as Ad5 and Ad2 serotypes including 19 (86.3 %) Ad5 and 3 (13.7 %) Ad2.
Conclusions:
This study reveals that adenoviruses with Ad5 predominance are important cause of respiratory tract infection in children.
